XSelect HSS PFP XP Column
XSelect HSS PFP XP Column

ACQUITY UPLC HSS PFP柱为分析方法开发科学家提供了独特、有力的选择性工具(与直链烷烃固定相相比)。五氟苯基(PFP:Pentafluorophenyl,或又称全氟苯基)配体提供多重保留机制包括:氢键作用、偶极-偶极作用、芳香性(π-π)作用,以及疏水(反相)作用,当试图分离异构性的、芳香性的、或是卤代的化合物特别有用。


含氟固定相为常规C18提供了互补的色谱分离选择性,特别是当需要分析碱性或平面芳香族化合物时。XSelect HSS PFP和CSH氟苯基色谱柱具有相同配体,但是不同的颗粒基体提供了不同的保留特性。



品名型号编号
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 2.1 mm X  100 mm, 1/pkg 186006175
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 3 mm X 30 mm,  1/pkg 186006176
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 3 mm X 50 mm,  1/pkg 186006177
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 3 mm X 75 mm,  1/pkg 186006178
XSelect HSS  PFP XP Column 100Å, 2.5 µm, , 3 mm X 100 mm,  1/pkg 186006179
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 2.1 mm X 30 mm,  1/pkg 186006172
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 2.1 mm X 50 mm,  1/pkg 186006173
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 2.1 mm X 75 mm,  1/pkg 186006174
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 2.1 mm X 75 mm,  3/pkg 176002644
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 2.1 mm X 100 mm,  3/pkg 176002645
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 3 mm X 30 mm,  3/pkg 176002646
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 3 mm X 50 mm,  3/pkg 176002647
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 2.1 mm X 30 mm,  3/pkg 176002642
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 2.1 mm X 50 mm,  3/pkg 176002643
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 3 mm X 75 mm,  3/pkg 176002648
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 3 mm X 100 mm,  3/pkg 176002649
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 4.6 mm X 30 mm,  1/pkg 186006180
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 4.6 mm X 50 mm,  1/pkg 186006181
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 4.6 mm X 75 mm,  1/pkg 186006182
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 4.6 mm X 100 mm,  1/pkg 186006183
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 3 mm X 150 mm,  3/pkg 176002904
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 3 mm X 150 mm,  1/pkg 186006746
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 2.1 mm X 150 mm,  3/pkg 176002903
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 4.6 mm X 150 mm,  1/pkg 186006747
XSelect HSS  PFP XP Column 100Å, 2.5 µm, 2.1 mm X 150 mm,  1/pkg 186006745


LC–tandem mass spectrometry method for the simultaneous determination of metformin and sitagliptin in human plasma after ion-pair solid phase extraction

A reversed-phase liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) method was established for simultaneous determination of two oral hypoglycemic drugs metformin (MET) and sitagliptin (STG) in human plasma. The analytes were extracted from 50
μL human plasma by ion-pair solid phase extraction using sodium lauryl sulphate on Phenomenex Strata-X (30 mg/1 mL) cartridges. The chromatographic separation was accomplished on XSelect HSS CN (150 × 4.6 mm, 5 μm) column using mobile phase consisting of methanol-8.0 mM ammonium formate in water, pH 4.5 (80:20, v/v) under isocratic condition. Tandem MS detection was performed on a triple quadrupole spectrometer equipped with an electrospray ionization source, operated in the positive mode. Multiple reaction monitoring (MRM) was used to quantify the analytes following transitions, m/z 130.1 60.1 and m/z408.3 235.1 for MET and STG respectively. The method displayed acceptable linearity in the concentration range of 4.00–3200 ng/mL for MET and 1.00–800 ng/mL for STG. The intra-batch and inter-batch precisions were ≤5.1% and accuracy ranged from 96.5 to 103.3% for both the drugs. The mean recovery of MET and STG obtained from spiked plasma samples was 82.5% and 90.4% respectively with minimal matrix interference. Both the drugs were found to be stable under all mandatory storage conditions. The validated method was successfully applied to a clinical pharmacokinetic study for a fixed-dose tablet formulation containing 500 mg MET and 50 mg STG in 16 healthy volunteers.


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