因能在pH 1-12范围内操作，这些三键键合式烷基柱能够利用pH的能力对可离子化化合物的保留、选择和灵敏度施加巨大影响，同时还能在低pH和高pH条件下保持优异的柱稳定性。相比于XBridge BEH C18填料， XBridge BEH C8填料适合分析弱极性物质里极性稍强的一类物质。
BEH颗粒能够显著改善色谱峰形的关键因素是其在合成工艺中 采用了亚乙基桥结构。亚乙基桥连接相邻的两个硅醇基，这 不仅增加了颗粒强度，同时也减少了游离的硅醇基位点，从 而减少硅醇基与样品间不利的相互作用力。传统的方法(例如 大量的封端(excessive end capping))仅限于封端试剂的空间位阻 效应及在活性位点上连接配体。这样，残余的游离硅醇基暴 露后就会导致峰展宽和峰拖尾。亚乙基桥可减少游离的硅醇 基数量，使分子空间排布最适于配体键合及封端。控制这一 过程可有助于XBridge色谱柱得到卓越的峰形。
色谱柱在低pH流动相中柱寿命差的主要原因是键合相在酸性 条件下发生水解。XBridge填料采用沃特世专有的、先进的键 合技术与封端技术，可以在低pH条件下表现出良好的配体稳 定性与色谱重现性。通过加速酸性稳定性测试，我们发现与传统填料相比，XBridge C18色谱柱几乎没有发生保留丧失或峰 形下降的情况，同时具有极佳的柱寿命，而这一特性并不是 通过对固定相进行立体位阻的技术手段所获得的。
Arsenic trioxide (As2O3) has been found to display anticancer activity against many types of tumors and has been developed into an anticancer drug in clinical treatments. Sphingolipids are membrane lipids that participate in many signal transduction pathways. In this paper, the changes in sphingolipids of the human multiple myeloma cell line U266 and the gastric cancer cell line MGC-803 treated with arsenic trioxide were investigated using an HPLC-ESI–MS/MS method. Analytes were separated by an XBridge BEH C8 column used for Cer, HexCer, LacCer and SM chromatographic separation, and a Capcell PAK MG II C18 column was used for Sph, dhSph, S1P and dhS1P chromatographic separation and gradient elution with acetonitrile–water containing 0.1% formic acid as a mobile phase. A tandem mass spectrometer QTrap in SRM mode was employed in combination with RPLC as a detector for quantitative analysis. The ceramide/sphingolipid internal standard (IS) mixture was used to quantify the levels of sphingolipids. The distributions of sphingolipids were found to be different in the human
品名 | 编号 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 2.1 mm X 100 mm, 1/pkg | 186003048 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 2.1 mm X 150 mm, 1/pkg | 186003049 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 1 mm X 100 mm, 1/pkg | 186003178 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 1 mm X 150 mm, 1/pkg | 186003179 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 1 mm X 50 mm, 1/pkg | 186003177 |
XBridge BEH C8 Column, 130Å, 5 µm, 3 mm X 100 mm, 1/pkg | 186003191 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 4.6 mm X 250 mm, 1/pkg | 186003963 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 2.1 mm X 50 mm, 1/pkg | 186003047 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 2.1 mm X 30 mm, 1/pkg | 186003046 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 4.6 mm X 30 mm, 1/pkg | 186003184 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 4.6 mm X 75 mm, 1/pkg | 186003185 |
XBridge BEH C8 Column, 130Å, 5 µm, 2.1 mm X 150 mm, 1/pkg | 186003013 |
XBridge BEH C8 Column, 130Å, 5 µm, 2.1 mm X 30 mm, 1/pkg | 186003187 |
XBridge BEH C8 Column, 130Å, 5 µm, 2.1 mm X 50 mm, 1/pkg | 186003011 |
XBridge BEH C8 Column, 130Å, 5 µm, 2.1 mm X 100 mm, 1/pkg | 186003012 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 3 mm X 30 mm, 1/pkg | 186003182 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 3 mm X 50 mm, 1/pkg | 186003050 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 3 mm X 150 mm, 1/pkg | 186003052 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 3 mm X 100 mm, 1/pkg | 186003051 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 4.6 mm X 100 mm, 1/pkg | 186003054 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 4.6 mm X 50 mm, 1/pkg | 186003053 |
XBridge BEH C8 Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1/pkg | 186003055 |
XBridge BEH C8 Column, 130Å, 5 µm, 4.6 mm X 250 mm, 1/pkg | 186003018 |
XBridge BEH C8 Column, 130Å, 5 µm, 4.6 mm X 150 mm, 1/pkg | 186003017 |
XBridge BEH C8 Column, 130Å, 5 µm, 4.6 mm X 100 mm, 1/pkg | 186003016 |
XBridge BEH C8 Column, 130Å, 5 µm, 4.6 mm X 50 mm, 1/pkg | 186003015 |
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