HILIC技术越来越多的被用作HPLC方法开发策略中的一部分。作为传统反相色谱技术的补充，XBridge HILIC柱分离不仅保留高极性化合物，还能够提供巨大的选择性差异，获得与反相分离时完全相反的洗脱顺序。拓宽的PH使用范围1-9，便于液相与质谱方法开发。HILIC保留机制是一个包含液液分配、离子交换与氢键的复杂过程，可增强极性分析物的保留。当使用极性固定相，并且流动相的乙腈浓度高于80%时，化合物将会得到很好的保留。
为方法开发提供互补选择性
一种高效的方法开发策略可同时使用XBridge HILIC色谱柱及XBridge 反相色谱柱来筛选分析物的选择性及保留性。在一些情况 下，使用该方法可产生不同的洗脱顺序或更佳的保留性，这 一特性有利于开发极性分析物的分离方法。
增强极性碱性化合物的保留
HILIC保留机制是一个包含液液分配、离子交换与氢键的复杂 过程，可增强极性分析物的保留。当使用极性固定相，并 且流动相的乙腈浓度高于80%时，化合物将会得到很好的保留。
提高质谱分析的灵敏度
HILIC的使用已经越来越普及，这主要是由于人们广泛采用质 谱仪作为检测器，并且需要提高对极性分析物的定量灵敏 度。不同于使用高比例水相流动相的反相分析方法，HILIC分 析方法中主要使用高比例乙腈流动相。含有高比例有机相的 流动相比较容易去溶剂化，从而增强离子强度及质谱响应。
人血浆百草枯高效液相色谱-串联质谱法的建立与评价
建立测定人血浆百草枯浓度的高效液相色谱-串联质谱法并评价.方法 用甲醇蛋白质沉淀法对血浆样品预处理后,以乙腈-水(含200 mmol/L甲酸铵和0.1％甲酸)为流动相梯度进样,流速为0.4 mL/min.通过色谱柱(Waters XBridge BEH HILIC,2.5 μm,2.1 mm×100 mm)分离样品.以ESI正离子模式、多反应离子监测(MRM)模式监测百草枯(m/z186.1→171.1作为定量离子对).用该方法检测临床患者血浆百草枯浓度,用ROC曲线评价血浆百草枯浓度和百草枯中毒严重指数(SIPP)对临床结局的判断价值.结果 血浆百草枯浓度在50～ 10 000 ng/mL时,线性关系良好(R2=0.997),最低定量下限为50 ng/mL.低(100 ng/mL)、中(2 000 ng/mL)、高(8 000 ng/mL)3个浓度水平质控品的不精密度均符合要求,且无基质效应.处理后样品室温放置6h内稳定,样本反复冻融3次对结果无影响.31例中毒患者SIPP为17.76(0.30～90.91)h·mg/L,死亡组SIPP高于存活组(P＜0.05).SIPP的ROC曲线下面积为0.889,最佳临床临界值为11.679 h·mg/L.结论 本法灵敏、准确、快速、特异性好,适用于临床检测百草枯中毒患者.
XBridge BEH HILIC Column, XP Column, 130Å, 2.5 µm, 2.1 mm X 150 mm, 3/pkg | 176002887 |
XBridge BEH HILIC Column, 130Å, 3.5 µm, 4.6 mm X 100 mm, 1/pkg | 186004440 |
XBridge BEH HILIC Column, 130Å, 3.5 µm, 4.6 mm X 150 mm, 1/pkg | 186004441 |
XBridge BEH HILIC Method Validation Kit, 130Å, 2.5 µm, 2.1 mm X 150 mm, 3/pkg | 186006779 |
XBridge BEH HILIC Column, 130Å, 5 µm, 2.1 mm X 30 mm, 1/pkg | 186004443 |
XBridge BEH HILIC Column, 130Å, 5 µm, 2.1 mm X 50 mm, 1/pkg | 186004444 |
XBridge BEH HILIC Column, 130Å, 5 µm, 2.1 mm X 150 mm, 1/pkg | 186004446 |
XBridge BEH HILIC Method Validation Kit, 130Å, 2.5 µm, 3 mm X 100 mm, 3/pkg | 186006224 |
XBridge BEH HILIC Column, 130Å, 5 µm, 2.1 mm X 100 mm, 1/pkg | 186004445 |
XBridge BEH HILIC Column, 130Å, 5 µm, 3 mm X 100 mm, 1/pkg | 186004448 |
XBridge BEH HILIC Column, 130Å, 5 µm, 3 mm X 50 mm, 1/pkg | 186004447 |
XBridge BEH HILIC Method Validation Kit, 130Å, 2.5 µm, 2.1 mm X 50 mm, 3/pkg | 186006221 |
XBridge BEH HILIC Method Validation Kit, 130Å, 2.5 µm, 2.1 mm X 100 mm, 3/pkg | 186006222 |
XBridge BEH HILIC Column, 130Å, 5 µm, 4.6 mm X 250 mm, 1/pkg | 186004454 |
XBridge BEH HILIC Column, 130Å, 5 µm, 4.6 mm X 100 mm, 1/pkg | 186004452 |
XBridge BEH HILIC Column, 130Å, 5 µm, 4.6 mm X 150 mm, 1/pkg | 186004453 |
XBridge BEH HILIC Column, 130Å, 3.5 µm, 2.1 mm X 50 mm, 1/pkg | 186004432 |
XBridge BEH HILIC Column, 130Å, 5 µm, 4.6 mm X 30 mm, 1/pkg | 186004450 |
XBridge BEH HILIC Column, 130Å, 5 µm, 4.6 mm X 50 mm, 1/pkg | 186004451 |
XBridge BEH HILIC Column, 130Å, 3.5 µm, 2.1 mm X 30 mm, 1/pkg | 186004431 |
XBridge BEH HILIC Column, 130Å, 3.5 µm, 3 mm X 100 mm, 1/pkg | 186004436 |
XBridge BEH HILIC Column, 130Å, 3.5 µm, 3 mm X 50 mm, 1/pkg | 186004435 |
XBridge BEH HILIC Column, 130Å, 3.5 µm, 2.1 mm X 150 mm, 1/pkg | 186004434 |
XBridge BEH HILIC Column, 130Å, 3.5 µm, 4.6 mm X 50 mm, 1/pkg | 186004439 |
XBridge BEH HILIC Column, 130Å, 3.5 µm, 4.6 mm X 30 mm, 1/pkg | 186004438 |
XBridge BEH HILIC VanGuard Cartridge, 130Å, 5 µm, 3.9 mm X 5 mm, 3/pkg | 186007789 |
XBridge BEH HILIC OBD Prep Column, 130Å, 5 µm, 19 mm X 250 mm, 1/pkg | 186004730 |
XBridge BEH HILIC Prep Guard Cartridge, 130Å, 5 µm, 30 mm X 10 mm, 1/pkg | 186006896 |
XBridge BEH HILIC Method Validation Kit, XP Column, 130Å, 2.5 µm, 3 mm X 50 mm, 3/pkg | 186006223 |
XBridge BEH HILIC Method Validation Kit, 130Å, 2.5 µm, 4.6 mm X 50 mm, 3/pkg | 186006225 |
XBridge BEH HILIC Method Validation Kit, 130Å, 2.5 µm, 4.6 mm X 100 mm, 3/pkg | 186006226 |
XBridge BEH HILIC OBD Prep Column, 130Å, 5 µm, 50 mm X 50 mm, 1/pkg | 186004732 |
XBridge BEH HILIC VanGuard Cartridge, 130Å, 3.5 µm, 3.9 mm X 5 mm, 3/pkg | 186007786 |
XBridge BEH HILIC OBD Prep Column, 130Å, 5 µm, 30 mm X 250 mm, 1/pkg | 186004731 |
XBridge BEH HILIC OBD Prep Column, 130Å, 5 µm, 50 mm X 150 mm, 1/pkg | 186004734 |
XBridge BEH HILIC OBD Prep Column, 130Å, 5 µm, 50 mm X 100 mm, 1/pkg | 186004733 |
XBridge BEH HILIC VanGuard Cartridge, 130Å, 5 µm, 2.1 mm X 5 mm, 3/pkg | 186007787 |
XBridge BEH HILIC OBD Prep Column, 130Å, 5 µm, 50 mm X 250 mm, 1/pkg | 186004735 |
XBridge BEH HILIC XP Column, 130Å, 2.5 µm, 2.1 mm X 100 mm, 3/pkg | 176002581 |
XBridge BEH HILIC VanGuard Cartridge, 130Å, 3.5 µm, 2.1 mm X 5 mm, 3/pkg | 186007784 |
XBridge BEH HILIC Method Validation Kit, 130Å, 2.5 µm, 3 mm X 150 mm, 3/pkg | 186006780 |
XBridge BEH HILIC Method Validation Kit, 130Å, 2.5 µm, 4.6 mm X 150 mm, 3/pkg | 186006781 |
XBridge BEH HILIC OBD Prep Column, 130Å, 5 µm, 30 mm X 150 mm, 1/pkg | 186004729 |
XBridge BEH HILIC OBD Prep Column, 130Å, 5 µm, 30 mm X 100 mm, 1/pkg | 186004728 |
XBridge BEH HILIC OBD Prep Column, 130Å, 5 µm, 30 mm X 50 mm, 1/pkg | 186004727 |
XBridge BEH HILIC OBD Prep Column, 130Å, 5 µm, 19 mm X 150 mm, 1/pkg | 186004726 |
XBridge BEH HILIC OBD Prep Column, 130Å, 5 µm, 19 mm X 100 mm, 1/pkg | 186004725 |
XBridge BEH HILIC Prep Guard Cartridge, 130Å, 5 µm, 19 mm X 10 mm, 1/pkg | 186004723 |
XBridge BEH HILIC XP Column, 130Å, 2.5 µm, 2.1 mm X 100 mm, 1/pkg | 186006079 |
XBridge BEH HILIC Prep Guard Cartridge, 130Å, 5 µm, 10 mm X 10 mm, 1/pkg | 186004720 |
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