XSelect HSS C18 柱是超高效通用性C18色谱柱,用于提供对碱性分析物的优异峰形、提高保留(相对于BEH C18柱),以及在低pH条件下的较长柱寿命。XSelect HSS C18 柱所表现出的选择性与保留性与大多数现代全封端的硅胶C18色谱柱色谱行为较为相近。
沃特世很清楚颗粒基质在色谱选择性和保留特性方面所起的作用:没有单独一种颗粒基体能够满足所有的色谱分析挑战。 另外,键合到不同基体上的同一配体也能产生各种不同的色谱结果。高强度硅胶 [HSS] 技术的开发专门用于与疏水性更 强的 BEH 和 CSH 颗粒的色谱性能相互补。与含亚乙基桥的 BEH 和 CSH 颗粒相比,HSS 颗粒更强的硅醇基活性(100% 硅胶) 可提供明显的色谱优势,包括增加极性化合物的保留和显著的选择性差异。另外,正如名字所示,HSS 颗粒被设计和合 成用于可承受 UPLC 压力所需的机械强度。
Development and validation of new RP-HPLC method for the determination of gliclazide in tablet dosage form
A new sensitive and economic analytical method for the analysis of gliclazide in tablet dosage form was developed and validated using commercial HPLC instrument with modern ultra-performance liquid chromatography column. Samples were analysed using Agilent 1100 Series RP-HPLC equipped with a binary pump and auto injector. Samples were injected into Xselect HSS C18 XP column (2.1 × 100 mm, 2.5 μm), 2.5 μm) and the mobile phase consisted of triethylamine buffer 0.3% (pH = 3 adjusted using phosphoric acid) and acetonitrile in the ratio of 50:50 v/v. The flow rate was 0.4 mL/min and the DAD detector was used for the detection and the wavelength was 229 nm. The method was validated according to ICH guidelines. The retention time for gliclazide peak was 3.437 ± 0.41 min with the total run time of 6 min with total solvent consumption of 2.4 mL per run. The method was found linear over the range 0.1-10 μg/mL with coefficient of determination R² of 0.9997. The recovery was 98.09-100.19 %, and the method showed high precision and repeatability. Degradation pathway of gliclazide was studied following different types of stress degradation which are base, acid, UV, and oxidization. All validated parameters were in the acceptable range of ICH requirements. In conclusion, a new, rapid, sensitive, and economic method was developed and validated for the analysis of gliclazide in tablet dosage form. © 2017, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.
品名 | 编号 |
XSelect HSS C18 Column, , 100Å, 3.5 µm, 1 mm X 150 mm, 1/pkg | 186006378 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 3 mm X 30 mm, 1/pkg | 186004765 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 3 mm X 50 mm, 1/pkg | 186004766 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 4.6 mm X 100 mm, 1/pkg | 186004767 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 4.6 mm X 150 mm, 1/pkg | 186004768 |
XSelect HSS C18 Column, 100Å, 5 µm, 2.1 mm X 100 mm, 1/pkg | 186006392 |
XSelect HSS C18 Column, 100Å, 5 µm, 2.1 mm X 50 mm, 1/pkg | 186006391 |
XSelect HSS C18 Column, 100Å, 5 µm, 2.1 mm X 150 mm, 1/pkg | 186006393 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 3 mm X 75 mm, 1/pkg | 186005642 |
XSelect HSS C18 Column, , 100Å, 5 µm, 2.1 mm X 30 mm, 1/pkg | 186006390 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 1 mm X 50 mm, 1/pkg | 186006376 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 1 mm X 100 mm, 1/pkg | 186006377 |
XSelect HSS C18 Column, 100Å, 5 µm, 3 mm X 150 mm, 1/pkg | 186006398 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 3 mm X 150 mm, 1/pkg | 186004763 |
XSelect HSS C18 Column, 100Å, 5 µm, 3 mm X 100 mm, 1/pkg | 186006397 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 3 mm X 100 mm, 1/pkg | 186004762 |
XSelect HSS C18 Column, 100Å, 5 µm, 2.1 mm X 150 mm, 3/pkg | 186006411 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 2.1 mm X 100 mm, 1/pkg | 186006383 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 2.1 mm X 75 mm, 1/pkg | 186006382 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 2.1 mm X 50 mm, 1/pkg | 186006381 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 2.1 mm X 30 mm, 1/pkg | 186006380 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 4.6 mm X 75 mm, 1/pkg | 186006387 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 4.6 mm X 50 mm, 1/pkg | 186004772 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 4.6 mm X 30 mm, 1/pkg | 186004771 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 2.1 mm X 150 mm, 1/pkg | 186006384 |
XSelect HSS C18 Column, 100Å, 3.5 µm, 4.6 mm X 250 mm, 1/pkg | 186004770 |
XSelect HSS C18 Column, 100Å, 5 µm, 4.6 mm X 100 mm, 1/pkg | 186006403 |
XSelect HSS C18 Method Validation Kit, 100Å, 3.5 µm, 4.6 mm X 100 mm, 3/pkg | 186006409 |
XSelect HSS C18 Method Validation Kit, 100Å, 2.5 µm, 2.1 mm X 50 mm, 3/pkg | 186006251 |
XSelect HSS C18 Method Validation Kit, 100Å, 2.5 µm, 2.1 mm X 150 mm, 3/pkg | 186006794 |
XSelect HSS C18 Column, 100Å, 5 µm, 4.6 mm X 30 mm, 1/pkg | 186006401 |
XSelect HSS C18 Column, 100Å, 5 µm, 4.6 mm X 75 mm, 1/pkg | 186006402 |
XSelect HSS C18 Method Validation Kit, 100Å, 2.5 µm, 3 mm X 150 mm, 3/pkg | 186006795 |
XSelect HSS C18 Intelligent Speed (IS) Column, 100Å, 5 µm, 4.6 mm X 20 mm, 1/pkg | 186006400 |
XSelect HSS C18 Method Validation Kit, 100Å, 2.5 µm, 4.6 mm X 150 mm, 3/pkg | 186006796 |
XSelect HSS C18 Column, 100Å, 5 µm, 3 mm X 250 mm, 1/pkg | 186006399 |
XSelect HSS C18 Column, 100Å, 5 µm, 3 mm X 50 mm, 1/pkg | 186006396 |
XSelect HSS C18 Column, XP Column, 100Å, 2.5 µm, 3 mm X 30 mm, 3/pkg | 176002622 |
XSelect HSS C18 Intelligent Speed (IS) Column, 100Å, 3.5 µm, 2.1 mm X 20 mm, 1/pkg | 186006379 |
XSelect HSS C18 Column, 100Å, 5 µm, 4.6 mm X 50 mm, 1/pkg | 186004852 |
XSelect HSS C18 Column, 100Å, 5 µm, 4.6 mm X 250 mm, 1/pkg | 186004775 |
XSelect HSS C18 Method Validation Kit, 100Å, 3.5 µm, 4.6 mm X 150 mm, 3/pkg | 186006410 |
XSelect HSS C18 Method Validation Kit, 100Å, 5 µm, 4.6 mm, X 250 mm, , 3/pkg | 186006416 |
XSelect HSS C18 Method Validation Kit, 100Å, 5 µm, 4.6 mm X 100 mm, 3/pkg | 186006414 |
XSelect HSS C18 Method Validation Kit, 100Å, 5 µm, 4.6 mm X 150 mm, 3/pkg | 186006415 |
XSelect HSS C18 Column, 100Å, 5 µm, 4.6 mm X 150 mm, 1/pkg | 186004773 |
相关产品