五氟苯基(PFP：Pentafluorophenyl，或又称全氟苯基)配体提供多重保留机制包括：氢键作用、偶极-偶极作用、芳香性(π-π)作用，以及疏水(反相)作用，当试图分离异构性的、芳香性的、或是卤代的化合物特别有用。
含氟固定相为常规C18提供了互补的色谱分离选择性，特别是当需要分析碱性或平面芳香族化合物时。XSelectHSS PFP和CSH氟苯基色谱柱具有相同配体，但是不同的颗粒基体提供了不同的保留特性
XSelectTM HSS(高强度硅胶颗粒)系列HPLC色谱柱一直在不断地 扩展其选择范围，现在新增一种五氟苯基[PFP]键合相。该新 款XSelect HSS PFP色谱柱是目前稳定性好且具重现性且可扩展的以硅胶颗粒为基质的PFP LC色谱柱。其HSS基质及PFP 配体为您提供真正的PFP性能，同时不牺牲峰形，也不会有保留时间不重现的问题。
Objective To develop a stable LC-MS/MS method for the determination of iopamidol in human plasma and evaluate the performance of the method. Methods Deuterium isotope was used as internal standard. Plasma samples were pretreated by direct precipitation,separated by XSelectTMHSS PFP column and equally eluted by 10% acetonitrile solution containing 0.1% fomic acid and 5 mmol/L nonafluoropentanoic acid. The detection was performed by ionic scanning with positive-ion electrospray and the results were analyzed by multiple reaction monitoring( MRM) mode. According to the CLSI EP10-A and CLSI C62-A standards,the linear range,accuracy,precision,specificity,matrix effect and stability of the method were evaluated. Results The linear range of iopamidol determined by LC-MS/MS was 1 to 1 000 μg I/mL( calculated with iodine content). The deviations between the determined results and the theoretical target values were from -6.1% to 7.5% in low,medium and high concentrations of plasma samples( 3,50 and 800 μg I/m L).The repeatability was expressed as imprecision with CV less than 6.6%( 4.6% to 6.6%). The intermediate precision was expressed with CV less than 8.5%( 6.6% to 8.5%). The recoveries were from 92.5% to 95.9%. The matrix effects of the method were from 88.8% to 95.2%. The results of the method showed excellent selectivity,since hemolysis and lipemia did not affect the results. In terms of this method,plasma samples had better kept at room temperature for no more than 48 hours and repeatedly freeze-thaw from -80 ℃ for no more than 2 times,so that iopamidol could almost still be stably detected. Iopamidol was also stable in extracted samples at 4℃ in the autosampler for 72 hours. Conclusion A reliable LC-MS/MS method for determination of iopamidol in human plasma was established and evaluated.
品名 | 规格 | 编码 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 1 mm X 150 mm, 1/pkg | 186005844 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 2.1 mm X 100 mm, 1/pkg | 186005849 |
XSelect HSS PFP Column | 100Å, 5 µm, 2.1 mm X 150 mm, 1/pkg | 186005872 |
XSelect HSS PFP Column | 100Å, 5 µm, 2.1 mm X 100 mm, 1/pkg | 186005871 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 3 mm X 30 mm, 1/pkg | 186005852 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 3 mm X 50 mm, 1/pkg | 186005853 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 3 mm X 75 mm, 1/pkg | 186005854 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 3 mm X 100 mm, | 186005855 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 3 mm X 150 mm, 1/pkg | 186005856 |
XSelect HSS PFP Column | 100Å, 5 µm, 3 mm X 50 mm, 1/pkg | 186005875 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 4.6 mm X 30 mm, 1/pkg | 186005858 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 4.6 mm X 50 mm, 1/pkg | 186005859 |
XSelect HSS PFP Column | 100Å, 5 µm, 3 mm X 100 mm, 1/pkg | 186005877 |
XSelect HSS PFP Column | 100Å, 5 µm, 3 mm X 150 mm, 1/pkg | 186005878 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 4.6 mm X 250 mm, 1/pkg | 186005863 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 4.6 mm X 150 mm, 1/pkg | 186005862 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 4.6 mm X 100 mm, 1/pkg | 186005861 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 4.6 mm X 75 mm, 1/pkg | 186005860 |
XSelect HSS PFP Column | 100Å, 5 µm, 4.6 mm X 30 mm, 1/pkg | 186005881 |
XSelect HSS PFP Column | 100Å, 5 µm, 4.6 mm X 100 mm, 1/pkg | 186005884 |
XSelect HSS PFP Column | 100Å, 5 µm, 4.6 mm X 150 mm, 1/pkg | 186005885 |
XSelect HSS PFP Column | 100Å, 5 µm, 4.6 mm X 250 mm, 1/pkg | 186005886 |
XSelect HSS PFP Column | 100Å, 5 µm, 2.1 mm X 30 mm, 1/pkg | 186005868 |
XSelect HSS PFP Column | 100Å, 5 µm, 2.1 mm X 50 mm, 1/pkg | 186005869 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 2.1 mm X 75 mm, 1/pkg | 186005848 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 2.1 mm X 30 mm, 1/pkg | 186005846 |
XSelect HSS PFP Column | 100Å, 3.5 µm, 2.1 mm X 50 mm, 1/pkg | 186005847 |
XSelect HSS PFP Method Validation Kit | 100Å, 2.5 µm, 2.1 mm X 50 mm, 3/pkg | 186006815 |
XSelect HSS PFP Method Validation Kit | 100Å, 2.5 µm, 3 mm X 50 mm, 3/pkg | 186006817 |
XSelect HSS PFP Method Validation Kit | 100Å, 2.5 µm, 2.1 mm X 100 mm, 3/pkg | 186006816 |
XSelect HSS PFP Method Validation Kit | 100Å, 2.5 µm, 4.6 mm X 100 mm, 3/pkg | 186006274 |
XSelect HSS PFP Method Validation Kit | 100Å, 2.5 µm, 4.6 mm X 50 mm, 3/pkg | 186006273 |
XSelect HSS PFP Method Validation Kit | 100Å, 2.5 µm, 3 mm X 100 mm, 3/pkg | 186006818 |
XSelect HSS PFP Method Validation Kit | 100Å, 2.5 µm, 2.1 mm X 150 mm, 3/pkg | 186006803 |
XSelect HSS PFP Method Validation Kit | 100Å, 2.5 µm, 3 mm X 150 mm, 3/pkg | 186006804 |
XSelect HSS PFP Method Validation Kit | 100Å, 2.5 µm, 4.6 mm X 150 mm, 3/pkg | 186006805 |
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