2004年，分离科学由于超高效液相色谱技术（Ultra-Performance Liquid Chromatography，UPLC®）的问世而发生了巨大的革命，在仪器和色谱柱方面的显著进步实现了液相色谱在分离度、灵敏度和速度方面的巨大跨越。一体化设计的ACQUITY UPLC®系统和ACQUITY UPLC色谱柱允许分析人员使用亚2微米颗粒色谱柱，以满足和克服分析试验室所面临的挑战，使分析科学家们更成功，业务收益更大、产出更多。
CSH技术的优势包括：
l 对于碱性化合物的优越峰形
l 提高载量
l 更换流动相pH后柱再平衡速度快
l 改善的批次之间重现性
l 在低pH和高pH条件下优异的稳定性
CSH颗粒技术也具有HPLC粒径产品(2.5,3.5和5微米)，对应于XSelectCSH HPLC柱家族，这确保在HPLC和UPLC技术平台之间方法可无缝转换。
ACQUITY UPLC CSH C18柱克服了大多数现代C18柱在低离子强度流动相条件下(如0.1%甲酸体系)因载量所导致的峰形差问题。因带有严格控制下的、低水平的正表面电荷，ACQUITY UPLC CSH C18柱为碱性化合物提供峰形，而无需使用离子对试剂。
目的建立超高效液相-紫外光电二极管阵列检测-串联四级杆-飞行时间质谱(UPLC-DAD/Q-TOF-MS)法分析左金丸化学成分。方法分析采用Waters ACQUITY UPLC CSH C18色谱柱(2.1 mm×100 mm,1.7μm);柱温20℃;流动相0.1%甲酸-0.1%甲酸乙腈,梯度洗脱;体积流量0.2 m L/min;检测波长222 nm;飞行时间质谱,正离子扫描模式。通过对照品比对,以及质谱、光谱数据进行分析。结果从中鉴定出19种成分,其中11种属于黄连,8种属于吴茱萸。结论该方法快速高效,可用于左金丸的质量控制。
品名 | 编号 |
ACQUITY - Xselect CSH C18 Method Transfer Kit, 130Å, 1.7 - 3.5 µm, 2.1 - 4.6 mm X 100 - 150mm, 2/... | 186005535 |
ACQUITY - Xselect CSH C18 Method Transfer Kit, 130Å, 1.7 - 3.5 µm, 2.1 - 4.6 mm X 50 - 100mm, 2/p... | 186005532 |
ACQUITY PREMIER CSH C18 Column 1.7 µm, 2.1 x 50 mm, 1/pk | 186009460 |
ACQUITY PREMIER CSH C18 Column 1.7 µm, 2.1 x 100 mm, 1/pk | 186009461 |
ACQUITY PREMIER CSH C18 Column 1.7 µm, 2.1 x 150 mm, 1/pk | 186009462 |
ACQUITY PREMIER CSH C18 Column with VanGuard FIT, 1.7 µm, 2.1 x 50 mm, 1/pk | 186009463 |
ACQUITY PREMIER CSH C18 Column with VanGuard FIT, 1.7 µm, 2.1 x 100 mm, 1/pk | 186009464 |
ACQUITY PREMIER CSH C18 Column with VanGuard FIT, 1.7 µm, 2.1 x 150 mm, 1/pk | 186009465 |
ACQUITY PREMIER CSH C18 VanGuard FIT Cartridge, 1.7 µm, 2.1 x 5 mm, 3/pk | 186009466 |
ACQUITY PREMIER Peptide CSH C18 Column, 130Å, 1.7 µm, 2.1 x 150 mm, 1/pk | 186009489 |
ACQUITY PREMIER Peptide CSH C18 Column, 130Å, 1.7 µm, 2.1 x 100 mm, 1/pk | 186009488 |
ACQUITY PREMIER Peptide CSH C18 Column, 130Å, 1.7 µm, 2.1 x 50 mm, 1/pk | 186009487 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 1 mm X 100 mm, 1/pkg | 186005293 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 3 mm X 75 mm, 1/pkg | 186005623 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pkg | 186005298 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 30 mm, 1/pkg | 186005295 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 1/pkg | 186005296 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 3 mm X 30 mm, 1/pkg | 186005299 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 30 mm, 3/pkg | 176002139 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 1 mm X 150 mm, 3/pkg | 176002138 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 3 mm X 50 mm, 1/pkg | 186005300 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 1 mm X 150 mm, 1/pkg | 186005294 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 1 mm X 100 mm, 3/pkg | 176002137 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 3 mm X 150 mm, 1/pkg | 186005302 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 1 mm X 50 mm, 3/pkg | 176002136 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 1 mm X 50 mm, 1/pkg | 186005292 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 3 mm X 100 mm, 1/pkg | 186005301 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 75 mm, 1/pkg | 186005620 |
ACQUITY UPLC CSH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg | 186005572 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 3 mm X 150 mm, 3/pkg | 176002146 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 3 mm X 100 mm, 3/pkg | 176002145 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 3/pkg | 176002140 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 3 mm X 30 mm, 3/pkg | 176002143 |
ACQUITY UPLC CSH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg | 186006016 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 3 mm X 50 mm, 3/pkg | 176002144 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg | 176002141 |
ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg | 176002142 |
ACQUITY UPLC CSH C18 VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pkg | 186005303 |
ACQUITY UPLC CSH C18 Method Validation Kit, 130Å, 1.7 µm, 3 mm X 100 mm, 3/pkg | 186005574 |
ACQUITY UPLC CSH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 50 mm, 3/pkg | 186005571 |
ACQUITY UPLC CSH C18 Method Validation Kit, 130Å, 1.7 µm, 3 mm X 50 mm, 3/pkg | 186005573 |
ACQUITY UPLC Peptide CSH C18 Column, 130Å, 1.7 µm, 1 mm X 100 mm, 1/pkg | 186006934 |
ACQUITY UPLC Peptide CSH C18 Column Kit, 130Å, 1.7 µm, 1 mm X 50 mm, 1/pkg | 176003061 |
ACQUITY UPLC Peptide CSH C18 Column Kit, 130Å, 1.7 µm, 1 mm X 100 mm, 1/pkg | 176003062 |
ACQUITY UPLC Peptide CSH C18 Column Kit, 130Å, 1.7 µm, 1 mm X 150 mm, 1/pkg | 176003063 |
ACQUITY UPLC Peptide CSH C18 Column Kit, 130Å, 1.7 µm, 2.1 mm X 50 mm, 1/pkg | 176003064 |
ACQUITY UPLC Peptide CSH C18 Column Kit, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pkg | 176003065 |
ACQUITY UPLC Peptide CSH C18 Column Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pkg | 176003066 |
ACQUITY UPLC Peptide CSH C18 Column Kit, Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3... | 176003068 |
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