ACQUITY UPLC BEH Shield RP18柱，除了C18链以外还包括一个亲水的氨基甲酸酯官能团(极性内嵌)，从而对碱性分析物提供优异的峰形。该色谱柱也提供相对于直链烷基色谱柱的其它选择性。
1.7 µm的亚乙基桥杂化颗粒[BEH]是UPLC®技术得以实现的关键要素之一。该产品目前提供三种不同的粒径[130Å、200Å和300Å]以及几种用于反相色谱和亲水作用色谱的键合相，适用于从小分子分析到大分子生物制药分析的各类应用。杂化颗粒技术因其固有的化学稳定性而能采用更宽的pH范围[pH 1-12]，从而可为方法开发提供一种通用且可靠的分离技术。
UPLC法测定地黄叶及块根中的梓醇和毛蕊花糖苷
目的比较地黄叶及块根中梓醇和毛蕊花糖苷的含有量.方法地黄叶及块根甲醇提取液的分析采用ACQUITY UPLC BEH Shield RP18色谱柱(2.1 mm×100 mm,1.7μm);柱温35℃;流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;体积流量0.2 m L/min;检测波长210 nm.结果梓醇含有量由高到低,依次为地黄嫩叶(149.22 mg/g)老叶(49.29 mg/g)块根(33.33 mg/g)干叶(15.53 mg/g);毛蕊花糖苷含有量依次为地黄老叶(10.27 mg/g)干叶(10.03 mg/g)嫩叶(9.56 mg/g)块根(0.28 mg/g).结论地黄嫩叶中梓醇的含有量约为老叶的3倍,但毛蕊花糖苷的含有量略低于老叶.地黄叶存放一年后,毛蕊花糖苷的减少量比梓醇小,表明前者稳定性高于后者.而且,这两种成分在叶中的含有量均比在块根中更高.
品名 | 编号 |
ACQUITY - XBridge BEH Shield RP18 Method Transfer Kit, 130Å, 1.7 - 3.5 µm, 2.1 - 4.6 mm X 50 - 100... | 186004965 |
ACQUITY - XBridge BEH Shield RP18 Method Transfer Kit, 130Å, 1.7 - 5 µm, 2.1 - 4.6 mm X 50 - 150mm... | 186004959 |
ACQUITY - XBridge BEH Shield RP18 Method Transfer Kit, 130Å, 1.7 - 3.5 µm, 2.1 - 4.6 mm X 100 - 15... | 186004972 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 1 mm X 100 mm, 3/pkg | 176000875 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 1 mm X 50 mm, 3/pkg | 176000874 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 1 mm X 150 mm, 1/pkg | 186003373 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 1 mm X 100 mm, 1/pkg | 186002852 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 1 mm X 50 mm, 1/pkg | 186002851 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 1 mm X 150 mm, 3/pkg | 176001045 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 3/pkg | 176000876 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 2.1 mm X 30 mm, 1/pkg | 186003909 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pkg | 186003376 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 3 mm X 100 mm, 1/pkg | 186004669 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pkg | 186002854 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 1/pkg | 186002853 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg | 176000877 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 3 mm X 30 mm, 1/pkg | 186004667 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 3 mm X 50 mm, 1/pkg | 186004668 |
ACQUITY UPLC BEH Shield RP18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg | 186006021 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg | 176001049 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 3 mm X 150 mm, 3/pkg | 176001805 |
ACQUITY UPLC BEH Shield RP18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 50 mm, 3/pkg | 186004048 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 3 mm X 50 mm, 3/pkg | 176001803 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 3 mm X 100 mm, 3/pkg | 176001804 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 3 mm X 30 mm, 3/pkg | 176001802 |
ACQUITY UPLC BEH Shield RP18 VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pkg | 186003977 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 2.1 mm X 30 mm, 3/pkg | 176001309 |
ACQUITY UPLC BEH Shield RP18 Method Validation Kit, 130Å, 1.7 µm, 3 mm X 100 mm, 3/pkg | 186004696 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 3 mm X 150 mm, 1/pkg | 186004670 |
ACQUITY UPLC BEH Shield RP18 Column, 130Å, 1.7 µm, 3 mm X 75 mm, 1/pkg | 186005610 |
ACQUITY UPLC BEH Shield RP18 Method Validation Kit, 130Å, 1.7 µm, 3 mm X 50 mm, 3/pkg | 186004695 |
ACQUITY UPLC BEH Shield RP18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg | 186004049 |
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