五氟苯基(PFP:Pentafluorophenyl,或又称全氟苯基)配体提供多重保留机制包括:氢键作用、偶极-偶极作用、芳香性(π-π)作用,以及疏水(反相)作用,当试图分离异构性的、芳香性的、或是卤代的化合物特别有用。
含氟固定相为常规C18提供了互补的色谱分离选择性,特别是当需要分析碱性或平面芳香族化合物时。XSelectHSS PFP和CSH氟苯基色谱柱具有相同配体,但是不同的颗粒基体提供了不同的保留特性。
ACQUITY UPLC HSS PFP色谱柱为三键键合、非封端的五氟苯基(PFP)化学特性。由于综合了多种保留机理,包括芳香(π-π)作用、氢键作用、偶极-偶极作用和疏水作用等,ACQUITY UPLC HSS PFP色谱柱理想用于平面芳香族化合物、位置异构体、卤代化合物以及极性分析物。
目的建立同时测定人血浆中卡马西平,拉莫三嗪,氯硝西泮,地西泮及其代谢物奥沙西泮浓度的方法.方法采用超高效液相色谱-质谱联用法(UPLC-MS/MS),以磺胺甲噁唑(SMZ)为内标,血浆经甲醇直接沉淀后进样分析.色谱柱为Waters ACQUITY UPLC HSS PFP柱(2.1 mm×100 mm,1.8μm),流动相为0.1%甲酸的5 mmol·L-1乙酸铵水溶液-0.1%甲酸的甲醇溶液(0~5 min,35:65→10:90),流速为0.2 mL·min-1.电喷雾离子源,正离子多反应监测扫描分析,卡马西平,拉莫三嗪,氯硝西泮,地西泮和奥沙西泮的离子对分别为m/z 237.0→194.06,m/z 255.98→144.95,m/z 316.01→270.0,m/z 285.04→193.07和m/z 287.02→241;内标磺胺甲噁唑的离子对为m/z 253.96→91.97.结果卡马西平,拉莫三嗪,氯硝西泮,地西泮和奥沙西泮血药浓度分别在2.4~600 ng·mL-1(r=0.999 7),2.52~630 ng·mL-1(r=0.992 0),2.08~520 ng·mL-1(r=0.997 9),2.28~570 ng·mL-1(r=0.998 2),8.0~800 ng·mL-1(r=0.999 2)线性关系良好;最低检出限分别为0.24,0.63,0.52,0.57,3.2 ng·mL-1.日内,日间精密度均15%;提取回收率均70%,且RSD15%.结论该方法灵敏,快速,专属性强,可用于临床血药浓度测定及药动学研究.
品名 | 编号 |
ACQUITY - Xselect HSS PFP Method Transfer Kit, 100Å, 1.7 - 3.5 µm, 2.1 - 4.6 mm X 50 - 100mm, 1/pk... | 186005980 |
ACQUITY - Xselect HSS PFP Method Transfer Kit, 100Å, 1.8 - 3.5 µm, 2.1 - 4.6 mm X 100 - 150mm, 1/p... | 186005981 |
ACQUITY UPLC HSS PFP VanGuard Pre-column, 100Å, 1.8 µm, 2.1 mm X 5 mm, 3/pkg | 186005974 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 3 mm X 75 mm, 1/pkg | 186005971 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 3 mm X 50 mm, 1/pkg | 186005970 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 3 mm X 150 mm, 1/pkg | 186005973 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 3 mm X 100 mm, 1/pkg | 186005972 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 2.1 mm X 50 mm, 1/pkg | 186005965 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 2.1 mm X 75 mm, 1/pkg | 186005966 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 1 mm X 150 mm, 1/pkg | 186005963 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 2.1 mm X 30 mm, 1/pkg | 186005964 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 3 mm X 30 mm, 1/pkg | 186005969 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 2.1 mm X 100 mm, 1/pkg | 186005967 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 2.1 mm X 150 mm, 1/pkg | 186005968 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 1 mm X 100 mm, 1/pkg | 186005962 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 1 mm X 50 mm, 1/pkg | 186005961 |
相关产品