合成寡核苷酸和DNA片段被应用于迅速发展的应用领域，包括作为主体或杂交探针用于治疗性制剂。沃特世寡核苷酸分离技术(OST：Oligonucleotide SeparationTechnology)基于BEH杂化颗粒的反相色谱柱，以及Gen-Pak™离子交换柱，应对各种高分辨分析与实验室规模分离挑战所需，包括涉及各种DNA和RNA品种。
寡核苷酸分析柱 — 异乎寻常的高分辨率
沃特世寡核苷酸色谱柱装有键合了C18的第二代杂化技术BEH颗粒。对去三苯甲基(detritylated，或称脱保护)的合成寡核苷酸样品的分离，基于成熟的离子对反相色谱法。沃特世提供1.7 µmUPLC颗粒或2.5 µm HPLC颗粒，装填以各种不同色谱柱规格，从而灵活满足各种实验室规模分离或分析的不同需求，并能实现异乎寻常的样品分辨率和卓越的色谱柱使用寿命。此外，沃特世的制造和质控测试程序，有助于确保批次之间与柱之间的性能的一致性，而无论应用的难度有多高。
■分离效率相当于或优于PAGE、CGE、或离子交换HPLC方法
■可从去三苯甲基(脱保护)的全长产物中分辨出失败序列
■可放大的柱规格，满足实验室规模的分离需求
■超长的色谱柱使用寿命，降低单次分析或分离成本
■经MassPREP OST标准品质控测试，帮助确保性能稳定
Waters oligonucleotide analysis solutions start with our award-winning ACQUITY UPLC systems and industry-leading oligonucleotide columns, which together deliver the ultimate in high-resolution chromatographic performance. Add to this our many detection options, including tunable UV, photodiode array, and fluorescence detectors, as well as nominal mass and high-resolution mass spectrometry equipment—all optimized for UPLC®—and you have a comprehensive set of tools to meet all of your oligonucleotide analysis needs.
This application notebook includes 24 application notes, illustrating work using Waters fit-for-purpose analytical solutions for oligonucleotides, and is organized by:
UPLC-MS for oligonucleotides
UPLC-HRMS for oligonucleotides
UPLC-HDMS for oligonucleotides
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